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A thin layer angiogenesis assay: A modified basement matrix assay for assessment of endothelial cell differentiation
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The volumes of matrix recommended for these assays (approximately 150 μl/cm2 ) are costly, limit working distances for microscopy, and require cell detachment for subsequent molecular analysis. Here we describe the development and validation of a thin-layer angiogenesis (TLA) assay for assessing the angiogenic potential of endothelial cells that overcomes these limitations. |