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Research on extraction of isoflavones from soybean germ
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In this paper, the total isoflavones were extracted from soybean germ. The five factors that influence the isoflavones extraction process including solvent concentration, extraction temperature, extraction time, extraction pH and the material/solvent ratio were optimized. The content of isoflavones in soybean germ material is from 1.8 to 2 percent. After extraction with optimized conditions, we obtain the total crude isoflavones extract that contains 3.8% of isoflavones. | Vietnam Journal of Science and Technology 56 4A 2018 61-68 RESEARCH ON EXTRACTION OF ISOFLAVONES FROM SOYBEAN GERM Do Thi Hoa Vien Dao Thi Trang School of Biotechnology and Food Technology Hanoi University of Science and Technology No. 1 Dai Co Viet Ha Noi Email vien.dothihoa@hust.edu. vn Received 20 September 2018 Accepted for publication 13 October 2018 ABSTRACT Isoflavones in soybean including genistein daidzein glycitein and their acetyl and malonyl derivatives have week estrogenic activity therefore they are called phytoestrogen. There are many papers publishing the research results about isoflavones from soybean seed and soybean sprout. Isoflavones are found to balance estrogenic hormone and support woman to prevent menopause symptoms. They have other precious biological activities such as anti - cancer anti -oxidant and anti - osteomalacia. On the market we find many functional foods of isoflavones from soybean seed and soybean sprout. Soybean germ is the most abundant isoflavones in different part of soybean seed and the isoflavones content in soybean germ higher than that in the whole soybean seed about 5 times. In this paper the total isoflavones were extracted from soybean germ. The five factors that influence the isoflavones extraction process including solvent concentration extraction temperature extraction time extraction pH and the material solvent ratio were optimized. The content of isoflavones in soybean germ material is from 1.8 to 2 percent. After extraction with optimized conditions we obtain the total crude isoflavones extract that contains 3.8 of isoflavones. Keywords extraction purification analysis isoflavones phytoestrogen soybean germ soygerm . 1. INTRODUCTION Soybean is a valuable crop which is abundant in Vietnam. Soybean provides an ideal source of vegetable protein containing essential amino acids with well-balanced ratio and high biological absorption. Moreover soybean is a predominant source of isoflavones the main class of .