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A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice

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Microinjection of clustered regulatory interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-related RNA and DNA into fertilized eggs is a novel approach for creating gene-modified mice. | A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice