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Serosurveillance of bovine brucellosis using RBPT and c-ELISA and comparative evaluation of test performance

The present study was conducted to evaluate the presence of Brucella infection amongst the randomly selected cattle and buffalo’s population in and around Chennai. The Rose Bengal Plate Test (RBPT) and Bru Alert® Brucella Antibody c-ELISA was employed to screen 355 sera samples (295 from cattle and 60 from buffalo) for the presence of Brucella antibody. The overall positivity of 8.73 % (31/355), 10.14 % (36/355) in bovine population, within this 9.15 % (27/295), 11.19 % (33/295) among white cattle and 6.66% (4/60), 5 % (3/60) among buffaloes by RBPT and c-ELSA respectively. Comparatively high seropositivity was found in >5 years age groups, females and exotic cross breed animals. | Serosurveillance of bovine brucellosis using RBPT and c-ELISA and comparative evaluation of test performance