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Isolation and characterization of multifunctional yeast as plant probiotics for better crop nutrition in pulses

Pulses being important source of protein and micronutrients find its place in national food security. Plant probiotic microbes play an important role in producing safe and nutritious pulses. In this work we characterized plant probiotic yeast from different sources and investigated its multifunctional activity in promoting the growth of black gram. Of the twenty isolates, two isolates one from the seeds of pomegranate (POY5) and the other one from grapes (GRY4) were selected for the study. Alignment results of the 18S rDNA sequences using ITS1 and ITS4 revealed POY5 as Pichia kudriavzevii and GRY4 as Issatchenkia terricola. Interestingly, both the isolates were positive for the plant growth promoting characteristics such as phosphorus solubilization, zinc solubilization, auxin, siderophore, ACC deaminase and hydrogen cyanide production that signify them as plant probiotics. Seed imbibition with the probiotic yeast recorded an increase in germination per cent of 9 and 8.8% by POY5 and GRY4 respectively over control. Significant difference in root and shoot length of black gram seedlings treated with probiotic yeast were recorded. It is concluded that these multi-functional yeast could be exploited as bioformulation for better crop nutritional security in black gram. | Isolation and characterization of multifunctional yeast as plant probiotics for better crop nutrition in pulses