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Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

A rapid and improved micropropagation protocol was developed for Curcuma aromatica, a threatened aromatic medicinal plant, using rhizome sprout as the explant. Stepwise optimization of different plant growth regulators, carbon sources, and basal media was adopted to establish an efficient micropropagation protocol. | Turkish Journal of Biology Turk J Biol (2013) 37: 698-708 © TÜBİTAK doi:10.3906/biy-1212-11 http://journals.tubitak.gov.tr/biology/ Research Article Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant 1,5 2 3 Shamima Akhtar SHARMIN , Mohammad Jahangir ALAM , Md. Mominul Islam SHEIKH , 4 5 6 Rashed ZAMAN , Muhammad KHALEKUZZAMAN , Sanjoy Chandra MONDAL , 7 6 1, Mohammad Anwarul HAQUE , Mohammad Firoz ALAM , Iftekhar ALAM * 1 Division of Applied Life Sciences (BK21 Program), College of Agriculture and Life Science, Gyeongsang National University, Jinju, Republic of Korea 2 Department of Bioscience (Integrated Bioscience Section), Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan 3 Division of Environmental Forest Sciences, College of Agriculture and Life Science, Gyeongsang National University, Jinju, Republic of Korea 4 Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, Gwangju, Republic of Korea 5 Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh 6 Department of Botany, University of Rajshahi, Rajshahi, Bangladesh 7 Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh Received: 06.12.2012 Accepted: 14.06.2013 Published Online: 08.10.2013 Printed: 04.11.2013 Abstract: A rapid and improved micropropagation protocol was developed for Curcuma aromatica, a threatened aromatic medicinal plant, using rhizome sprout as the explant. Stepwise optimization of different plant growth regulators, carbon sources, and basal media was adopted to establish an efficient micropropagation protocol. When cytokinins, such as benzyl amino purine (BAP) or 6-(α,αdimethylallylamino)-purine (2iP), were used either singly or in combination with naphthalene acetic acid (NAA) for shoot induction and .